General Cell Lines and Cell Cultures
Genscript Corporation HEK293/EP1 Stable Cell Line
Recombinant HEK293 cells stably overexpress human prostaglandin E receptor 1 (PTGER1) on the surface and contain high levels of G protein G q to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/ADRB1/G15 Stable Cell
The -adrenergic receptors are linked to G proteins The -receptor has three known subtypes Beta-1 receptors primarily regulate myocardial tissue and affect the rate of contraction via impulse conduction Beta-2 receptors regulate smooth muscle tone and influence vascular and bronchiolar relaxation Beta-3 receptors are less well studied but are thought to primarily affect lypolysis and may have effects on cardiac inotropy (Greene Shepherd 2006) In the human heart beta (1)- and beta (2)AR are the most powerful physiologic mechanism to acutely increase cardiac performance Changes in betaAR play an important role in chronic heart failure (CHF) Thus due to increased sympathetic activity in CHF betaAR are chronically (over) stimulated and that results in beta (1) AR desensitization and alterations of down-stream mechanisms (Brodde OE 2006)
Genscript Corporation CHO-K1/CRF2/G15 Stable Cell L
Recombinant CHO-K1 cells stably overexpress human corticotropin releasing hormone receptor 2 (CRF2) on the surface and contain high levels of G protein G s to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/cyno PD-L2 Stable Cell
Programmed cell death 1 ligand 2 (PD-L2) is a protein that is encoded by the PDCD1LG2 gene in humans PDCD1LG2 has also been designated as CD273 (cluster of differentiation 273) Inhibitory molecules of the B7/CD28 family play a key role in the induction of immune tolerance in the tumor microenvironment The programmed death-1 receptor (PD-1) with its ligands PD-L1 and PD-L2 constitutes an important member of these inhibitory pathways PD-L2 expression was initially thought to be restricted to antigen-presenting cells such as macrophages and dendritic cells (DCs) However PD-L2 expression can be induced on a wide variety of other immune cells and nonimmune cells depending on micro environmental stimuli
Genscript Corporation CHO-K1/5-HT1A/G15 Stable Cell
The 5-Hydroxytryptamine receptor 1A (5-HT1A) is G sub i /sub -coupled GPCRs expressed in the cerebral cortex hippocampus septum amygdala and raphe nucelus with lesser amounts in the basal ganglia and thalamus Many functions of the central nervous system are influenced by 5-HT including sleep motor activity sensory perception arousal and appetite 5-HT1A ligands may prove to be therapeutic in the treatment of various disorders such as depression anxiety and schizophrenia
Genscript Corporation CHO-K1/ADORA2A/G15 Stable Cel
Recombinant CHO-K1 cells stably overexpress human adenosine A2A receptor (ADORA2A) on the surface and contain high levels of G protein G s to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/G15/CXCR1
Chemokine (C-X-C motif) receptor 1 (CXCR1) is a rhodopsin-like G protein-coupled receptor It is one of two high-affinity receptors for the CXC chemokine interleukin-8 (IL-8) a major mediator of immune and inflammatory responses The structure of human CXCR1 in a lipid bilayer should help to facilitate the discovery of new compounds that interact with GPCRs GenScript s human CXCR1-expressing stable subline is guaranteed to function properly in the calcium flux assay
Genscript Corporation CHO-K1/CysLT1/G15 Stable Cell
CysLT1 (Cysteinyl leukotriene receptor 1) is previous named as LTD4 receptor (leukotriene D4 receptor) It is a receptor for cysteinyl leukotrienes and has highest affinity to leukotriene D4 (LTD4) The receptor mediates contraction and proliferation of smooth muscle edema eosinophil migration and damage to the mucus layer in the lung caused by LTD4 A CysLT1 selective antagonist montelukast is used clinically in the treatment of asthma This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system The rank order of affinities for the leukotrienes is LTD4 LTE4 LTC4 LTB4
Genscript Corporation CHO-K1/mouse PD-L1 Stable Cell
Recombinant CHO-K1 cells stably overexpress mouse programmed cell death 1 ligand 1 (PD-L1) on the surface The surface expression of PD-L1 is validated by FACS analysis
Genscript Corporation CHO-K1/Cyno CD47 Stable Cell L
This gene encodes a membrane protein which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin and it may play a role in membrane transport and signal transduction This gene has broad tissue distribution and is reduced in expression on Rh erythrocytes Alternatively spliced transcript variants have been found for this gene
Genscript Corporation CHO-K1/ADRA2C/G15 Stable Cell
The adrenoceptors ADRA2C is expressed in the brain kidney aorta lung skeletal muscle heart and spleen ADRA2C knockout mice exhibited disrupts presynaptic inhibition of noradrenaline release at low stimulation frequencies
Genscript Corporation CHO-K1/Mouse VISTA Stable Cell
V-domain Ig suppressor of T cell activation (VISTA) is a potent negative regulator of T-cell function that is expressed on hematopoietic cells VISTA levels are heightened within the tumor microenvironment in which its blockade can enhance antitumor immune responses in mice In humans blockade of the related programmed cell death 1 (PD-1) pathway has shown great potential in clinical immunotherapy trials
Genscript Corporation CHO-K1/mouse CD155 Stable Cell
CD155 commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5) is a type I transmembrane single-span glycoprotein and belongs to the nectins and nectin-like (Necl) subfamily CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV) an etiologic agent of the central nervous system disease poliomyelitis The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells CD155 may assist in an efficient humoral immune response generated within the intestinal immune system It s been shown that CD155 can be recognized and bind by DNAM-1 and CD96 which promotes the adhension migration and NK-cell killing Thus inducing cell-mediated tumor-specific immunity
Lonza Walkersville Mouse Embry Fibro Nufe Kit 1
The Nucleofector 2b System (or older generations I or II) works cell type specific kits each of them dedicated to an individual primary cell The Mouse Embryonic Fibroblast Nucleofector Kit 1 is one of our two kits suited for efficient transfection of primary mouse embyonic fibroblasts (MEF) Due to significant diversity between different MEF clones we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone The respective cell type-specific Optimized Protocol or recommendation is available under Instructions or in our Lonza Bioscience knowledge database In this Optimized Protocol the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of this cell type
Lonza Walkersville PNBM Primary Neuron Basal Med
Clonetics PNBM Primary Neuron Basal Medium (CC-3256) specifically developed for culturing embryonic primary rat and mouse neurons For successful culture of neurons basal media should be combined with growth factors (sold seperately) Basal medium and SingleQuots Growth Factors can be purchased seperately or in convenient PNGM BulletKit format (CC-4461)